Immunophenotyping.

نویسندگان

  • C C Stewart
  • S J Stewart
چکیده

This unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two-, three-, and four-color staining. Analysis of data acquired from cells stained by these procedures is detailed. A procedure is given for the detection of the location of nonviable cells so that they can be gated out of the analysis.

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عنوان ژورنال:
  • Current protocols in cytometry

دوره Chapter 6  شماره 

صفحات  -

تاریخ انتشار 2001